Written by International Journal of Pharmacology and Clinical Sciences, 2014, 3, 3, 61-67.
Published: September 2014
Type: Research Article
Authors: Hardik Soni, Ankur Shah, Arindam Paul, and Ghanshyam Patel
Author(s) affiliations:
Hardik Soni1*, Ankur Shah2, Arindam Paul2, Ghanshyam Patel1
1Vasu Research Centre, A Division of Vasu Healthcare Pvt. Ltd., 896/A, G.I.D.C., Makarpura, Vadodara, Gujarat-390010, India.
2Department of Pharmacology, Babaria Institute of Pharmacy, BIT education campus, Vadodara-Mumbai NH#8, Varnama , Vadodara, Gujarat – 391240, India.
Abstract
Background: This study was aimed to investigate anti-ulcer activity of a herbo-mineral formulation (Asecure capsule) in ethanol-induced acute gastric ulcer and acetic acid-induced chronic gastric ulcer. Materials and Methods: Absolute methanol (5 mL/kg, p.o.) was administered for development of acute gastric ulcer. In another model, 50% acetic acid was exposed to serosal surface of stomach to produce chronic gastric ulcer. For each experiment, animals were divided in to three groups where each group was consisting of six animals. Group-I, II and III were considered as Normal control, Disease control and test drug treated respectively. Herbo-mineral formula-tion (Asecure Capsule) was administered at 100 mg/kg/day, orally. Ulcer index, gastric wall mucus content, lipid perox-idation level in stomach tissue and tissue anti-oxidant parameters like superoxide dismutase, reduced glutathione and catalase enzyme activity were carried out for both experimental studies. Histopathology of stomach tissue was also performed. Statistical calculations were done by analysis of variance (ANOVA) followed by post hoc Bonferroni’s test. Results: Induction of ethanol and acetic acid caused significant alteration in ulcer index, gastric wall mucus content and lipid peroxidation level in comparison to normal control group whereas treatment of Asecure capsule showed sig-nificant cyto-protection and recovery against ethanol and acetic acid induced acute and chronic ulcer respectively. Treatment with Asecure capsule) also showed significant increase in level of antioxidant enzymes in comparison of disease control groups. Histopathology of stomach showed remarkable cyto-protection. Conclusion: On the basis of present data it can be concluded that Asecure capsule) possesses significant anti-ulcer activity against exposure to a noxious agent like ethanol and acetic acid. The observed effect be due to synergistic anti-oxidant property of ingredients of the herbo-mineral formulation.
Keywords: Anti-ulcer activity, Ethanol and acetic acid induced gastric ulcer, Herbo-mineral