Written by International Journal of Pharmacology and Clinical Sciences, 2015, 4, 3, 52-57.
DOI: 10.5530/ijpcs.4.3.4
Published: Sept 2015
Type: Original Article
Authors: Vishal Bhat, Arul Amuthan, Barizah Binti Mohamed Rosli, Nur Amirah Binti Khairuddin, and Fatin Shahira Binti Md. Isa
Author(s) affiliations:
Vishal Bhat,1 Arul Amuthan,1 Barizah Binti Mohamed Rosli,2 Nur Amirah Binti Khairuddin,2 Fatin Shahira Binti Md. Isa2
1Department of Pharmacology, Melaka Manipal Medical College and Center for Integrative Medicine and Research,Manipal University, Karnataka-576104, INDIA.
2MBBS Student, Melaka Manipal Medical College, Manipal University, Karnataka-576104, INDIA.
Abstract
Background: Kungiliya vennai and Kalchunna thailam are two indigenous herbal formulations used for healing wounds in Siddha medicine. Objectives: To evaluate the wound healing property of topically applied Kungiliya vennai (KV) and Kalchunna thailam (KT) on excision wounds in Sprague Dawley rats. Methods: Four groups of eight female Sprague Dawley rats in each group were used. Excision wounds of 300 mm² size were created under anesthesia using scissors. Group 1 served as a control with no drugs applied. Groups 2, 3, and 4 were treated with mupirocin, KV, and KT, respectively, daily until complete healing. Parameters observed were % wound contraction rate and epithelialization period. Biopsies of wounds for histology were taken on day 12 and after complete healing. Results were analyzed using one-way ANOVA followed by Tukey’s test (p < 0.05). Results: KT increased wound contraction rate only during initial days, but not significantly. KV treatment increased vascularity with tissue eosinophils compared to control rats. KV treatment also resulted in regeneration of adnexal skin structures. The epithelialization period was significantly hastened in KV and KT groups compared to the control group. Conclusion: Topical application of KV and KT promoted wound healing in rats. KV showed additional properties of regenerating adnexal structures such as hair follicles, sweat glands, and sebaceous glands.
Keywords: Calcium, Kungiliya Vennai, Shorea Robusta, Siddha Medicine, Wound